University at Buffalo, The State University of New York

Paez Laboratory

During the past five years Dr. Paez have found new and novel functions of voltage and store-operated Ca ++ channels as modulators of intracellular Ca ++ levels in oligodendrocytes. They have shown that these ion channels affect many Ca ++ dependent functions in oligodendrocyte progenitor cells (OPCs) as a consequence of their ability to modulate intracellular Ca ++ concentrations.

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Paez Research Overview

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Our research program focuses on brain development, studying the development of the oligodendroglial and astroglial cell lineages in the central nervous system in normal, mutant and transgenic mice. The primary focus in the laboratory is on ion channels that regulate specification, migration and differentiation of these glial cells. The oligodendrocyte generates CNS myelin, which is essential for normal nervous system function. Thus, investigating the regulatory and signaling mechanisms that control its differentiation and the production of myelin is relevant to our understanding of brain development and of adult pathologies such as multiple sclerosis. Astrocytes are the most abundant cell of the human brain. They perform many functions, including biochemical support of endothelial cells that form the blood brain barrier, provision of nutrients to the nervous tissue and a role in the repair and scarring process of the brain and spinal cord following traumatic injuries. Therefore, understanding the role of ion channels on astrocyte development and function could lead to novel approaches to intervene in neurodegenerative diseases and brain injuries in which inflammation and astrogliosis play a detrimental role.

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Faculty and Staff

Dr. Pablo M. Paez
Assistant Professor of Pharmacology & Toxicology

Dr. Pablo M. Paez is a Principal Investigator of the Hunter James Kelly Research Institute and Assistant Professor in Pharmacology and Toxicology in the School of Medicine and Biomedical Sciences, University at Buffalo...

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Veronica Cheli

Senior Research Scientist, HJKRI, Paez Lab

  • 2013-present Senior Research Scientist Hunter James Kelly Research Institute School of Medicine and Biomedical Sciences University at Buffalo, The State University of New York
  • 2011-2012 Staff Research Associated Intellectual and Developmental Disabilities Research Center David Geffen School of Medicine University of California at Los Angeles
  • 2006-2011 Postdoctoral Scholar Department of Human Genetics, David Geffen School of Medicine University of California at Los Angeles
  • 2000-2006 Academic Tutor/Teaching Assistant Laboratory of Neuroscience, Department of Cell Biology School of Pharmacy and Biochemistry University of Buenos Aires, Argentina.
  • 2000-2005 Research Assistant and Instructor Laboratory of Neuroscience, Department of Cell Biology School of Pharmacy and Biochemistry University of Buenos Aires, Argentina.
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    Vilma Spreuer

    Research Technician, HJKRI, Paez Lab

  • 2013-present Research Technician Step III Hunter James Kelly Research Institute School of Medicine and Biomedical Sciences University at Buffalo, The State University of New York
  • 1992-2012 Staff Research Associated Step II Intellectual and Developmental Disabilities Research Center David Geffen School of Medicine University of California at Los Angeles
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    Diara Santiago Gonzalez

    PhD student, HJKRI, Paez Lab

  • 2008 - 2012: Bachelor Degree in Chemistry, University of Puerto Rico, Rio Piedras Campus.
  • 2012 - present: PhD Student at the University at Buffalo, NY, USA, School of Medicine and Biomedical Sciences, Department of Pharmacology and Toxicology, Hunter James Kelly Research Institute.
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    Norma Zamora

    PhD student, HJKRI, Paez Lab

  • 2011 – 2013: BA Psychology, California State University, San Bernardino.
  • 2015 – Present: PhD Student in the Department of Pharmacology and Toxicology, University at Buffalo, School of Medicine and Biomedical Sciences.
  • Students

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    Tensing Lama, Master student, College of Art and Sciences

    Selected Publications

    • Cheli VT, Santiago Gonzalez DA, Smith J, Spreuer V, Murphy GG, Paez PM. L-type voltage-operated calcium channels contribute to astrocyte activation In vitro. Glia. 2016.
    • Guardia Clausi M, Paez PM, Pasquini LA, Pasquini JM. Inhalation of growth factors and apo-transferrin to protect and repair the hypoxic-ischemic brain. Pharmacol Res. 2016.
    • Levine J, Kwon E, Paez P, Yan W, Czerwieniec G, Loo JA, Sofroniew MV, Wanner IB, Paez PM. Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival. Glia. 2015.
    • Cheli V.T., Santiago Gonzalez D.A., Spreuer V., Paez P.M. (2015). Voltage-gated Ca++ entry promotes oligodendrocyte progenitor cell maturation and myelination in vitro. Experimental Neurology 265 (2015) 69-83.
    • Cheli VT, Santiago González DA, Lama TN, Spreuer V, Handley V, Murphy GG and Paez PM. Conditional Deletion of the L-Type Calcium Channel Cav1.2 in Oligodendrocyte Progenitor Cells Affects Postnatal Myelination in Mice. Journal of Neuroscience Oct 2016, 36 (42) 10853-10869.
    • Cheli VT, Santiago Gonzalez DA, Smith J, Spreuer V, Murphy GG, Paez PM. L-type voltage-operated calcium channels contribute to astrocyte activation In vitro. Glia. 2016.
    • Guardia Clausi M, Paez PM, Pasquini LA, Pasquini JM. Inhalation of growth factors and apo-transferrin to protect and repair the hypoxic-ischemic brain. Pharmacol Res. 2016.
    • Levine J, Kwon E, Paez P, Yan W, Czerwieniec G, Loo JA, Sofroniew MV, Wanner IB, Paez PM. Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival. Glia. 2015.
    • Cheli V.T., Santiago Gonzalez D.A., Spreuer V., Paez P.M. (2015). Voltage-gated Ca++ entry promotes oligodendrocyte progenitor cell maturation and myelination in vitro. Experimental Neurology 265 (2015) 69-83.
    • Paez PM, Cheli VT, Spreuer V, Handley V and Campagnoni AT (2012). Golli myelin basic proteins stimulate oligodendrocyte progenitor cell proliferation and differentiation in remyelinating adult mouse brain. Glia 60: 1078-1093.
    • Guardia Clausi M, Paez PM, Campagnoni AT, Pasquini LA and Pasquini JM (2012). Intranasal administration of apotransferrin protects and repairs the neonatal white matter after a cerebral hypoxic-ischemic event. Glia 60: 1540-1554.
    • Nobuta H, Ghiani CA, Paez PM, Spreuer V, Dong H, Korsak RA, Manukyan A, Li J, Sofroniew MV, Campagnoni AT, de Vellis J and Waschek JA (2012). Cross talk between reactive astrocytes and microglia protects myelin development after perinatal inflammatory insult. Annals of Neurology 72: 750-765.
    • Paez PM, Fulton DJ, Spreuer V, Handley V and Campagnoni AT (2011). Modulation of Canonical Transient Receptor Potential Channel 1 in the Proliferation of Oligodendrocyte Precursor Cells by the Golli Products of the Myelin Basic Protein Gene. J Neurosci. 31: 3625-3637.
    • Paez PM, Fulton DJ, Spreuer V, Handley V and Campagnoni AT (2010). Multiple kinase pathways regulate voltage-dependent Ca++ influx and migration in oligodendrocyte precursor cells. J Neurosci. 30: 6422-6433.
    • Fulton DJ, Paez PM, Fisher R, Handley V, Colwell CS and Campagnoni AT (2010). Regulation of L-type Ca++ Currents and Process Morphology In White Matter Oligodendrocyte Precursor Cells by Golli-Myelin Proteins. Glia 58: 1292-1303.
    • Paez PM, Fulton D, Colwell CS and Campagnoni AT (2009). Voltage-operated Ca++ and Na+ channels in the oligodendrocyte lineage. J Neurosci Res. 87: 3259-3266.
    • Paez PM, Fulton DJ, Spreuer V, Handley V, Campagnoni CW, Macklin WB, Colwell C and Campagnoni AT (2009). Golli myelin basic proteins regulate oligodendroglial progenitor cell migration through voltage-gated Ca++ influx. J Neurosci. 29: 6663-6676.
    • Paez PM, Fulton DJ, Spreuer V, Handley V, Campagnoni CW and Campagnoni AT (2009). Regulation of store-operated and voltage-operated Ca++ channels in the proliferation and death of oligodendrocyte precursor cells by golli proteins. ASN-Neuro 1(1): 25-41.
    • Paez PM, Spreuer V, Handley V, Feng JM, Campagnoni C and Campagnoni AT (2007). Increased Expression of Golli Myelin Basic Proteins Enhances Calcium Influx into Oligodendroglial Cells. J Neurosci. 27: 12690-12699.

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